TABLE 1.
Free energy parameters for CggR interaction with the DNA operator half-sites
|
D + OL ⇆ DOL
|
D + OR ⇆ DOR
|
2D + OR ⇆ D2OR
|
|||
|---|---|---|---|---|---|
| [FBP] (μM) | ΔG0 (kcal/mol) | Kd (nM) | ΔG0 (kcal/mol) | Kd (nM) | ΔG0 (kcal/mol) |
| 0 | 9.8 ± 0.2 | 52 | 14 (−0.6; +1.5) | 0.03 | 23 (−0.5; +6.5) |
| 2000 | 9.6 ± 0.2 | 73 | 11.9 ± 0.3 | 1.4 | |
D represents a CggR dimer. Confidence limits were determined from rigorous confidence limit testing at the 67% level (mean ± 1 SD). ΔG0 values consigned correspond to the dissociation reaction. Values indicated in italics are approximate, the very low Kd values compared to DNA concentration (1.7 nM) precluding more accurate determination.