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. 2007 Feb 9;92(9):3251–3259. doi: 10.1529/biophysj.106.089011

FIGURE 4.

FIGURE 4

Simultaneously acquired forward and backward SHG images in a 20-μm-thick spinal tissue slice. The forward signal was collected by a 60× water immersion objective. The forward and backward PMT detectors were of the same type and set at the same gain. (A and B) Simultaneously acquired forward and backward SHG images of fibrils inside spinal cord white matter. (C) Line analysis of a large fibril indicated by red lines in A and B. (D and E) Simultaneously acquired forward and backward SHG images of the same area as in A and B but at a different depth. (F) Line analysis of a small fibril indicated by red lines in D and E. The laser power at the sample was 7 mW. Scale bar, 10 μm.