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. 2007 Feb 9;92(9):3178–3194. doi: 10.1529/biophysj.106.097105

FIGURE 7.

FIGURE 7

Shape changes of egg PC-GUVs induced by the addition of EGCg at room temperature. (A) A prolate changed into a dumbbell, and then into two spheres connected by a narrow neck upon addition of 5 μM EGCg. The time after starting addition of EGCg solution through the micropipette is (1) 0 s, (2) 11 s, and (3) 14 s. After the addition of EGCg was stopped, the shape change was reversed. The time after stopping the addition of EGCg is (4) 3 s, (5) 8 s, (6) 11 s, and (7) 17 s. (B) A cylinder changed into a pearls-on-a-string upon addition of 1 μM EGCg. The time after starting addition of 1 μM EGCg solution through the micropipette is (1) 0 s, (2) 10 s, and (3) 18 s. After the addition of EGCg was stopped, the shape change was reversed. The time after stopping the addition of EGCg is (4) 2 s, (5) 15 s, and (6) 94 s. (C) A prolate changed into two spheres connected by a neck upon addition of 1 μM EGCg, and then the two spheres associated each other (4). The time after starting addition of 1 μM EGCg solution through the micropipette is (1) 0 s, (2) 11 s, (3) 18 s, and (4) 21 s. When EGCg was no longer added, the two associated spheres did not detach. The time after stopping the addition of EGCg is (5) 30 s and (6) 88 s. The bars in (A) and (C) correspond to 10 μm, and the bar in (B) corresponds to 10 μm.