Figure 1.

(A) Sequences of the 3′ end of rpoH (Upper) and the trpBA junction of rpoHBAZ (Lower). In the rpoHBAZ construct, the C-terminal Ala codon of rpoH was fused in-frame with the 5′ end of trpB at the SphI site, whereas the 5′ end of trpA is fused in-frame to codon 9 of lacZ at the BamHI site. The stop codon (TGA) of trpB and the initiation codon (ATG) of trpA are boxed. Amino acids in bold letters originate from the trpBA junction. (B) Schematic diagrams of rpoHBAZ and its derivatives constructed as described in Materials and Methods. Arrowheads indicate the position of the G123A mutation that enhances translation, and parentheses indicate the mutation or constructs containing the mutation. The expected fusion protein products for each construct are indicated to the right.