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Expression of A-LacZ fusion protein from MC4100 (λrpoHBAZ) and its derivatives
| Construct | β-gal activity of A-LacZ, Miller U |
|---|---|
| rpoHBAZ | 222.93 ± 10.10 |
| rpoHΔC17BAZ | 82.36 ± 2.40 |
| 123BAZ | 762.25 ± 25.78 |
| 123ΔC17BAZ | 81.77 ± 2.21 |
Cells were grown in M9 medium at 30°C to midlog phase and assayed for β-galactosidase activity.
