. Author manuscript; available in PMC: 2007 Apr 17.

Published in final edited form as: Dev Biol. 2006 Jul 28;299(1):206–220. doi: 10.1016/j.ydbio.2006.07.022

Table 1.

Changing of mitochondria distribution after electrical activation, IVF, enucleation or somatic cell nuclear transfer

Treatments^c	No. of oocytes examined	No. (%) of oocytes showing mitochondria distribution
		Cortical ooplasm			Perinuclear association
		Lipid^d	Clusters^e	Others^f	Negative^g	Positive^h
						Total	Ringsⁱ
IVM	24	14 (58)^a	9 (38)^a	1 (4)^a	24 (100)^a	0 (0)	0 (0)
Electrical activation	48	6 (13)^b	42 (88)^b	0 (0)	43 (90)^a	5 (10)^a	1 (2)^a
IVF	38	1 (3)^b	37 (97)^b	0 (0)	18 (47)^b	20 (53)^b	16 (42)^b
Enucleation	72	45 (63)^a	10 (14)^a	17 (24)^a	–	–	–
SCNT w/o Act.	29	18 (62)^a	11 (38)^b	0 (0)	11 (38)^b	18 (62)^b	0 (0)
SCNT followed by Act.	52	5 (10)^b	47 (90)^b	0 (0)	30 (58)^b	22 (42)^b	7 (13)^a

^{a, b}

Values with different superscripts within the column are significantly different (P<0.05).

IVM: in vitro maturation; Electrical activation: at 6 h after electrical activation with a 1.2 kV DC pulse; IVF: at 10–12 h after sperm insemination; Enucleation: at 3 h after removal of oocyte's spindles; SCNT w/o Act.: at 3 h after somatic cell nuclei transfer into enucleated oocytes; SCNT followed by Act.: at 6 h after electrical activation with a 1.2 kV DC pulse following somatic cell nuclear transfer into enucleated oocytes.

Localization of mitochondria associated with lipid droplets at the cortical ooplasm.

Formation of several sizes of mitochondrial clusters at the cortical ooplasm.

No or weak signals of mitochondria at the cortical ooplasm.

No signal of mitochondria was detected in the vicinity of nuclei formed after each treatment.

Mitochondria signals were observed in the vicinity of nuclei formed after each treatment.

ⁱ

Continuous rings of mitochondria were observed at the rim of pronuclei.