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. Author manuscript; available in PMC: 2008 Mar 15.
Published in final edited form as: Vet Immunol Immunopathol. 2007 Jan 9;116(1-2):1–12. doi: 10.1016/j.vetimm.2006.12.005

Fig. 3.

Fig. 3

Fig. 3

M. paratuberculosis induces nitrite production by infected macrophages, but addition of autologous T cells does not further increase nitrite production. Nitrites were measured in culture supernatants using the Griess reaction. Bars represent the mean±SEM from 8 animals. (A) Cells obtained from calves prior to inoculation with M. paratuberculosis. Blood-derived macrophages infected in vitro with M. paratuberculosis with and without added T cells produce similar levels of nitrites as infected cultures containing autologous unstimulated γδ T cells or unstimulated CD4+ T cells. Mptb = M. paratuberculosis. (B) Blood-derived macrophages and sorted T cells from lymph nodes draining sites of M. paratuberculosis inoculation at PID 60. Infected macrophages, cultured with or without autologous unstimulated or PPD-stimulated γδ T cells or CD4+ T cells, produce similar levels of nitrites. Note that nitrites in cultures containing PPD-stimulated CD4+ T cells are not increased compared to cultures without added T cells, despite the substantial IFN-γ produced by CD4+ T cell-containing cultures (Fig. 2B). Cultures containing uninfected macrophages (with or without added T cells) produce low to undetectable amounts of nitrites. PPD = purified protein derivative.