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. Author manuscript; available in PMC: 2008 Mar 15.
Published in final edited form as: Vet Immunol Immunopathol. 2007 Jan 9;116(1-2):1–12. doi: 10.1016/j.vetimm.2006.12.005

Fig. 5.

Fig. 5

Treatment of M. paratuberculosis-infected bovine macrophages with recombinant IFN-γ does not increase nitrite production or increase mycobacterial killing. (A) Data from one representative naïve animal; bars represent the mean±SEM from 3 experiments. Blood-derived macrophages (either uninfected or infected in vitro with M. paratuberculosis) were incubated with varying concentrations of recombinant rIFN-γ alone or in combination with 1 μg/mL E. coli LPS. Dashed horizontal line indicates the mean nitrite concentration from parallel uninfected macrophage cultures (n=3). * P<0.05 compared to infected cultures receiving 100 U/mL IFN-γ. **P<0.001 compared to infected cultures receiving 25 U/mL IFN-γ. (B) Live bacteria recovered from macrophage cultures 48 hours after in vitro infection. Infected cultures received varying concentrations of IFN-γ alone or in combination with 1 μg/mL E. coli LPS. Percent live bacteria indicates the percent of fluorescein-positive bacteria among total bacterial cells recovered from lysed infected macrophage cultures using the fluorescein diacetate procedure as described in Materials and Methods. Each point represents the mean±SEM from 2 naïve animals.