Table 3.

Precision and Accuracy of LC-MS-MS Analysis of Ginkgolides and Bilobalide

		Day-1			Day-2			Day-3
Compound	Actual amount (pg)	Measured amount (pg)	RSDa (%)	REb (%)	Measured amount (pg)	RSD (%)	RE (%)	Measured amount (pg)	RSD (%)	RE (%)	Inter-day RSD (%)
Ginkgolide A	650	689	2.82	6.00	696	3.76	7.07	693	2.27	6.58	0.51
	1600	1659	4.4	3.69	1573	0.61	−1.68	1658	3.88	3.65	3.03
	4000	3817	1.86	−4.57	3990	4.46	−0.24	3906	1.08	−2.35	3.17
Ginkgolide B	650	699	3.13	7.62	695	3.34	6.95	694	1.03	6.76	0.38
	1600	1689	1.87	5.58	1648	2.34	2.97	1644	4.54	2.75	1.50
	4000	3754	2.58	−6.16	4000	2.27	0.00	3888	3.00	−2.80	2.22
Ginkgolide C	650	676	2.58	3.94	702	2.60	8.08	638	2.76	−1.84	4.79
	1600	1671	3.74	4.44	1608	2.11	0.51	1650	2.34	3.14	1.95
	4000	3808	2.97	−4.80	4036	4.46	0.91	3849	2.47	−3.76	3.12
Ginkgolide J	650	673	2.85	3.60	637	1.45	−2.03	701	4.98	7.81	4.79
	1600	1646	3.63	2.85	1622	3.98	1.37	1728	4.63	8.01	3.34
	4000	3734	1.01	−6.65	4018	3.46	0.36	3954	2.57	−1.14	3.78
Bilobalide	650	667	2.40	2.67	696	3.93	7.07	631	4.17	−2.85	4.90
	1600	1591	4.47	−0.53	1588	4.97	−0.69	1713	4.46	7.08	4.37
	4000	3813	2.34	−4.68	3976	3.00	−0.60	3964	0.83	−0.91	2.32

^aRSD = relative standard deviation (n = 3)

^bRE = relative error