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Plot of the change in the relative fluorescence intensity as a function of time for a refolding jump from 6 M to 0.54 M urea at pH 7.80 and 15°C, measured on the Applied Photophysics SX.17MV stopped-flow unit. Tryptophan fluorescence was followed by using an excitation wavelength of 293 nm and by monitoring the emission for wavelengths >320 nm.
