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(a) Plot of the change in relative fluorescence intensity as a function of time for a refolding jump from 6 M to 0.54 M urea at pH 7.80 and 15°C, measured on the Applied Photophysics SX.17MV stopped-flow unit. Tryptophan fluorescence was monitored by an excitation wavelength of 293 nm and emission observed for wavelengths >320 nm. (b) Plot of the difference in absorbance on methotrexate binding as a function of time for a refolding jump from 6 M to 0.54 M urea, pH 7.80 and 15°C.
