Timing of interventions is shown in relation to ischemia. Ouabain was perfused at 10 μM. The c-Src kinase inhibitor PP2 (4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolol[3,4-d]pyrimidine, 2 μM) and the (PKCε) translocation inhibitor peptide (TIP, 5 μM) were given before, during and after ouabain. After ischemia, all hearts were reperfused with standard Krebs-Henseleit solution. * Except for TIP groups.