Left ventricular developed pressure (LVDP, upper panel) and end diastolic pressure (EDP, lower panel) were measured in Langendorff-perfused hearts submitted to no treatment (IR, □), ouabain (OIR, ▪), PP2 (PP2+IR, ▵) or PP2+ouabain (PP2+OIR, ▴). Values are means ± SEM of 5–7 independent experiments for each group. ** P<0.01 and *** P<0.001 vs. IR. ## P<0.01 and ### and P<0.001 vs. OIR.