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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Dev Biol. 2006 Aug 12;302(1):25–39. doi: 10.1016/j.ydbio.2006.08.023

Fig. 8. tbx-2::GFP expression is repressed by Notch signaling.

Fig. 8

(A) tbx-2::GFP 5.2kb translational reporter is de-repressed at the 8E stage when the ref-1 family is inactivated by feeding RNAi, but activated normally with glp-1 and lag-1 inactivation. TBX-2::GFP expression in 8E cell embryos (B, D, F H, J) and 16E cell embryos (C, E, G, I, K). WT embryos (B, C), compared to feeding RNAi of glp-1 (D,E), lag-1 (F,G), and ref-1members (H,I) shows de-repression of tbx-2::GFP at the 16E cell stage. pha-4(RNAi) reduces the number of TBX-2::GFP expressing cells (J,K). Error bars indicate SD. P values of tbx-2::GFP cell counts of RNAi embryos compared to wild type are glp-1 p= 0 .32, refs p = 0.001, lag-1 p = 0.0003, pha-4 p = 0.0000003.