TABLE 1.
Connexin Antibodies Used for Western Blotting and Immunohistochemistry*
| Antibody | Type | Epitope; Designation | Reference; Source |
|---|---|---|---|
| connexin26 | Monoclonal | C-terminus; 33–5800 | Nagy et al., 2001; Zymed |
| connexin26 | Polyclonal | C-terminus; 51–2800 | Nagy et al., 2001; Zymed |
| connexin29 | Polyclonal | C-terminus; 34–4200 | Li et al., 2002; Zymed |
| connexin30 | Monoclonal | C-terminus; 33–2500 | Rash et al., 2001; Zymed |
| connexin30 | Polyclonal | C-terminus; 71–2200 | Nagy et al., 1999; Zymed |
| connexin32 | Monoclonal | aa 235–246; 7C7 | Li et al., 1997 |
| connexin32 | Monoclonal | C-terminus; 35–8900 | Zymed |
| connexin32 | Monoclonal | Cytoplasmic loop; 13–8200 | Zymed |
| connexin32 | Polyclonal | Cytoplasmic loop; 71–0600 | Li et al., 1997; Zymed |
| connexin32 | Polyclonal | C-terminus; 34–5700 | Zymed |
| connexin43 | Monoclonal | C-terminus; 35–5000 | Zymed |
| connexin43 | Polyclonal | C-terminus; 71–0700 | Li et al., 1998; Zymed |
| connexin43 | Polyclonal | aa 346–363; 18A | Yamamoto et al., 1990a,b |
| connexin47 | Polyclonal | C-terminus; 36–4700 | Zymed |
*
All antibodies were raised against peptides corresponding to connexin sequences. Monoclonal Cx32 antibody 13–8200 and polyclonal 71–0600 are directed against the same sequence in the cytoplasmic loop region of Cx32. Monoclonal 35–8900 and polyclonal 34–5700 are directed against the same sequence in the C-terminus region of Cx32. Zymed, Zymed Laboratories, South San Francisco, CA.