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. Author manuscript; available in PMC: 2007 Dec 1.

Published in final edited form as: Mol Microbiol. 2006 Dec;62(6):1586–1600. doi: 10.1111/j.1365-2958.2006.05475.x

Aggregate formation on the light organ by V. fischeri. Newly hatched juvenile squid were inoculated with GFP-labeled bacteria. After 2 to 6 h, animals were stained with Cell Tracker Orange (red color) and the light organs examined by confocal microscopy. Representative images of aggregated V. fischeri cells at or near a light organ pore are shown. Animals were inoculated with (A) ES114 (KV1066), (B) rscS mutant cells (KV1339), (C) wild-type cells carrying the vector control (KV2685), (D and E) wild-type cells carrying rscS1 (KV2688), (F and G) rscS1-expressing sypN mutant cells (KV2689), and (H) sypN mutant cells carrying vector (KV2686). Arrows indicate pores of the light organ.

Aggregate formation on the light organ by V. fischeri. Newly hatched juvenile squid were inoculated with GFP-labeled bacteria. After 2 to 6 h, animals were stained with Cell Tracker Orange (red color) and the light organs examined by confocal microscopy. Representative images of aggregated V. fischeri cells at or near a light organ pore are shown. Animals were inoculated with (A) ES114 (KV1066), (B) rscS mutant cells (KV1339), (C) wild-type cells carrying the vector control (KV2685), (D and E) wild-type cells carrying rscS1 (KV2688), (F and G) rscS1-expressing sypN mutant cells (KV2689), and (H) sypN mutant cells carrying vector (KV2686). Arrows indicate pores of the light organ.