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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2007 Mar;13(3):277–292. doi: 10.1016/j.bbmt.2006.11.018

Figure 2.

Figure 2

Adoptive transfer of non-separated splenocytes provided a better vaccine-induced anti-tumor effect than T cells purified by positive selection using immunomagnetic sorting. Lethally irradiated A/J mice were transplanted with 107 syngeneic BM cells with or without (a) 5x106 positively-selected Thy1.2+ T cells, (b) 2x107 non-separated splenocytes, or (c) 4x107 non-separated splenocytes. One week after HSCT, the mice given adoptive T cell/spleen cell transfer were vaccinated twice weekly with 2x106 irradiated 4P-AGN2a cells. One week after the second vaccination (day 21 after HSCT) all mice were challenged with 5x106 viable AGN2a cells. The data represents the combined results of two or three separate experiments, and the groups consisted of 10-16 total mice per group.