Figure 2.

Effect of Dex on the phosphorylation state of major MAPKs and the JNK substrate c-Jun in basal and Dex treated sensitive and resistant CEM clones. (A) Cell clones CEM-C7-14 and CEM-C1-6 (both Dex-sensitive) as well as CEM-C1-15 (Dex-resistant) were treated with ethanol vehicle (C) or 1 μM Dex (D) for 20–24 hours. Cell lysates were evaluated by use of phospho-specific antibodies. Shown is a typical immunoblot for phosphorylated MAPK, from 4–5 independent experiments. (B) One of three immunoblots for phosphorylated c-Jun.