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Real-time RTPCR from testis and adrenal. Relative proportions of Sry mRNA in the testis and adrenal glands of SHR/y males (n = 5 per group). S26 ribosomal RNA was used as control transcript to normalize Sry levels. Mean ΔCT Sry (adrenal gland) = 11.54 +/- 0.30 and mean ΔCT Sry (testis) = 11.54 +/- 0.30. Fold difference, Sry (testis) compared Sry (adrenal gland) = 2-(7.88–11.54) = 12.6X.
