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. 2007 Apr 17;5(5):e102. doi: 10.1371/journal.pbio.0050102

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Cells were labeled and isolated from regenerating muscle as in Figure 2. Half of the cells were fixed immediately (Before Division). The other half (After Division) was cultured overnight to allow the cells to complete the current cell cycle and divide. The cells were then harvested and analyzed by FACS for Sca-1, the labeled (younger) template (CldU), and newly synthesized DNA (IdU). Gatings were on forward scatter (FSC) and IdU-labeled cells.

(A) A representative FACS plot of Sca-1 versus CldU is shown in the population before and after cell division.

(B) Quantitation of FACS plots as shown in (A) demonstrates the decrease in the percentage of Sca-1⁺ cells that have labeled template strands before and after cell division and the corresponding increase in the percentage of Sca-1⁻ cells having labeled template strands (n ≥ 3; a single asterisk [*] indicates p < 0.01).