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TREX1 exonuclease activity. Cell-free protein extracts were assayed for 3′ DNA exonuclease activity with a 3′ labeled poly(dA) substrate after partial purification by column chromatography on single-stranded DNA cellulose. GM0558 is a normal lymphoblastoid control. F39A is a lymphoblastoid cell line from a child with biallelic mutations in TREX1.
