Figure 3. .

Immunodetection showing no evidence that the IL-6 SR antibody preferentially binds to D358A. We spotted 10-mer, 11-mer, and 12-mer peptides from the IL-6R protein onto a cellulose array with 384 features and immunodetected with the IL-6 SR monoclonal antibody employed in the ELISA assay to study the subjects in the Health ABC Study. Three potential peptide spots from the 10-mers were detected, including an intense positive signal (spot #110 at amino acids 219–228) and two other positive signals (spot #68 at amino acids 135–144 and spot #144 at amino acids 287–296). No intense spots overlapped amino acid 358, which we spotted in both polymorphic forms and was included in numerous peptides. Results are from a 1-h exposure (similar results were obtained from an overnight exposure [not shown]).