Figure  2. .

Mutations in SIX5 affect SIX5-Eya1 binding. Yeast two-hybrid assays were performed to test the effect of the mutations on the ability of SIX5 to bind to Eya1. Cotransformation of GAL4BD-SIX5 and GAL4AD-Eya1D leads to strong lacZ expression. An >2-fold reduction in LacZ expression is seen when SIX5 proteins containing an A158T or a T552M mutation are cotransformed with Eya1D fusion proteins.