Table 3. .
FANCD2 Superamplicon Primers
| Forward | Reverse | |||||
| Superamplicon | Containing Exon(s) | Designation | Sequence(5′→3′) | Designation | Sequence(5′→3′) | PCR Product Size(bp) |
| I | 1 and 2 | hFANCD2_exon1_F | TATGCCCGGCTAGCACAGAA | hFANCD2_super_1_2_R | GGCCCACAGTTTCCGTTTCT | 4,346 |
| II | 3 | hFANCD2_super_3_3_F | GTGTCACGTGTCTGTAATCTC | hFANCD2_super_3_3_R | CTGGGACTACAGACACGTTTT | 2,323 |
| III | 7, 8, and 9 | hFANCD2_super_7_14_F | TGGGTTTGGTAGGGTAATGTC | hFANCD2_exon9_R | TACTCATGAAGGGGGGTATCA | 4,595 |
| IV | 10, 11, 12, 13, and 14 | hFANCD2_exon10_F | GCCCAGCTCTGTTCAAACCA | hFANCD2_super_7_14_R | TTAAGACCCAGCGAGGTATTC | 5,635 |
| V | 13, 14, 15, 16, and 17 | FA-D2, sup13-I17 F | CATGGCAGGAACTCCGATCTTG | FA-D2, sup13-I17 F | CTCCCTTAAAAGCTCAAAGCTCAAGTTC | 8,858 |
| VI | 19 and 20 | hFANCD2_super_19_22_F | ACGTAATCACCCCTGTAATCC | hFANCD2_exon20_R | TGACAGAGCGAGACTCTCTAA | 2,749 |
| VII | 21, 22, and 23 | FA-D2, 21_23, F | GCTTCTAGTCACTGTCAGTTCACCAG | FA-D2, 21_23, R | ACGTTGGCCAGAAAGTAATCTCAG | 2,518 |
| VIII | 23, 24, 25, and 26 | hFANCD2_super_23_29_F | GGCCTTGTGCTAAGTGCTTTT | hFANCD2_exon26_R | TCAGGGATATTGGCCTGAGAT | 3,252 |
| IX | 27, 28, and 29 | hFANCD2_exon27_F | GCATTCAGCCATGCTTGGTAA | hFANCD2_super_23_29_R | CACTGCAAACTGCTCACTCAA | 3,371 |
| X | 30 | hFANCD2_super_30_32_F | CCAAAGTACTGGGAGTTTGAG | hFANCD2_exon30_R | TACCCAGTGACCCAAACACAA | 2,186 |
| XVI | 31 and 32 | hFANCD2_exon31_F | CCATTGCGAACCCTTAGTTTC | hFANCD2_super_30_32_R | ACCCTGGTGGACATACCTTTT | 299 |
| XII | 33 and 34 | hFANCD2_super_33_36_F | GAGCAATTTAGCCTGTGGTTTT | hFANCD2_exon34_R | TATAGCAAGAGGGCCTATCCA | 3,457 |
| XIII | 35 and 36 | hFANCD2_exon35_F | TTAGACCGGGAACGTCTTAGT | hFANCD2_super_33_36_R | TCTGGGCAACAGAACAAGCAA | 2,040 |
| XIV | 43a | hFANCD2_super_43_44_F | AGGGTCCTGAGACTATATACC | hFANCD2_exon43a_R | AGCATGATCTCGGCTCACCA | 2,040 |
| XV | 44 | hFANCD2_exon44_F | CACCCAGAGCAGTAACCTAAA | hFANCD2_super_43_44_R | ACCATCTGGCCGACATGGTA | 464 |