Figure 1.

Stra13 is induced in response to DNA damage. (A) NIH3T3 cells were treated with etoposide or cisplatin and stimulated with retinoic acid 13 (Stra13) expression was analysed by western blot at the indicated time points. Untreated (UT) cells were used as controls. Membranes were reprobed with β-actin antibody. (B,C) NIH3T3 cells (B) and p53-null 10.1 T cells (C) were irradiated (10 Gy), and Stra13 expression was analysed by western blot at the indicated times. 10.1 T cells were analysed 2 h after irradiation. (D,E) Wild-type mice were exposed to ionizing radiation and killed after 2.5 or 6 h. Stra13 expression was analysed in the thymus (6 h) and spleen (2.5 h). Untreated mice were used as controls. Two mice were used for each dose.