Figure 4.
dap−/− nurse cells have reduced levels of the chromatin-bound MCM2–7 complex. (A, B) Wild-type and (C, D) dap−/− egg chambers stained with (A, C) αMCM2–7 antibody and (B, D) DAPI, and washed with low salt to reveal the nucleoplasmic pool of MCM2–7. (E, F) Wild-type and (G–H) dap−/− egg chambers stained with (E, G) αMCM2–7 antibody and (F, H) DAPI and washed with high salt to reveal chromatin-bound MCM2–7.