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Schematic representation of synaptotagmin constructs used for FRET studies of metal-dependent C2 domain rearrangement. Shaded boxes denote the regions composing the first and second C2 domains of synaptotagmin (C2A and C2B). The single tryptophan of the C2A domain (W259, energy donor) and the single cysteine of the C2B domain (C277), to which coumarin (energy acceptor) was conjugated, are shown.
