FIGURE 6.

Deletion analysis of the the yeast HRA1 gene. (A) The 10-nt deletion (ATCAGGGAAT, from 99,705 to 99,714) in the HRA1 gene is designed upstream of the sense DRS2 gene (bold) in yeast chromosome 1 and introduced into yeast cells (see Materials and Methods). (B) Growth of the wild-type strain, VS165CH1WT (triangles), and deletion strain, VS165CH1D (squares), after transfer from overnight galactose-containing medium to either galactose-containing (left panel) or glucose-containing (right panel) medium at T = 0 h. Yeast cells were cultured at 30°C, and cell densities were measured at times indicated.