Figure 6.

Dok-3 undergoes stimulation-dependent homo-oligomerization. Dok-3^−/− mutant cells were reconstituted with Dok-3/GFP and subsequently transfected with empty control vector (lanes 1 and 2) or expression constructs for either HA-tagged wild-type Dok-3 (lanes 3 and 4) or the indicated HA-tagged Dok-3 variants (lanes 5–12; for details, see Figure 3A). Lysates were subjected to anti-HA immunoprecipitation. Proteins obtained were analyzed by immunoblotting with antibodies to GFP (upper panel) and HA peptide tag (lower panel). Relative molecular masses of marker proteins are indicated on the left in kDa.