Fig 5.
Immunolocalization of cleaved-caspase-3 on whole-mount embryos. Embryos treated with 1 mM CdCl2, respectively, at blastula (A–C) and gastrula (E–G) stages for 15, 21, and 24 hours, incubated with anti-cleaved-caspase-3 antibody. Control embryos (34 and 44 hours of development, respectively) incubated with anti-cleaved-caspase-3 (D, H)