Fig 3.
Genetic interaction between SGT2 and YDJ1. Liquid cultures (optical density at 600 nm [OD600] ≈ 4 to 6) were diluted to OD600 = 0.1 with sterile water. Three microliters of cell suspension were then spotted on yeast extract/peptone/dextrose (YPD) plates with or without 2% formamide and were streaked. The growth phenotypes were subsequently examined. The mutations in ydj1Δsgt2Δ were verified by Western blot analysis (not shown). Left panel: Different yeast strains as indicated were grown on YPD plates at 30°C and 35°C for 2 days. The cells grown at 35°C were then shifted to 30°C, and they were allowed to grow for 2 more days. Although ydj1Δ cells appeared fully recovered, ydj1Δsgt2Δ cells did not recover as well. Right panel: The cells with the indicated mutations were allowed to grow on YPD plates with 2% formamide at 30°C and were photographed after 2 days. Two different isolates of ydj1Δsgt2Δ obtained through independent tetrad analysis were showed here