Figure 6.

IEC anoikis does involve the activation of caspase 1 family cysteine proteases. Cytosolic extracts were prepared and the fluorescence assay performed as described in Figure 5 ▶ but using instead YVAD-AMC (striped bars ). Cytosolic protein from the human monocytic cell line U937 stimulated with LPS was used as positive control. Enzymatic activity was inhibited by the addition of 150 μmol/L YVAD-CHO (solid bars).