Table 1.
Conventional PCR | |
---|---|
Primers | |
bcl-2 mbr | 5′-TTAGAGAGTTGCTTACGTGGCCTG-3′ |
bc-2 mcr | 5′-GACTCCTTTACGTGCTGGTACC-3′ |
JH | 5′-GGACTCACCTGAGGAGACGGTGACC-3′ |
Probes | |
bcl-2 mbr | 5′-CTGTTTCAACACAGACCC-3′ |
bcl-2 mcr | 5′-ACCAGGTNCCTTGGCCCCA-3′ |
Exonuclease-based PCR | |
Primers | |
bcl-2 mbr | 5′-TTAGAGAGTTGCTTTACGTGGCC-3′ |
bcl-2 mcr | 5′-CCTGGCTTCCTTCCCTCTGT-3′ |
JH | 5′-ACTCACCTGAGGAGACGGTGAC-3′ |
Fluorescent dye-labeled probes | |
bcl-2 mcr | 5′-FAM-TCTCTGIGGAGGAGTGGAAAGGAAGG-TAMR |
bcl-2 mbr | 5′-FAM-TTTCAACACAGACCCACCCAGAGCC-TAMRA |