Figure 7.

HGF affects the subcellular distribution of E-cadherin. Twenty-four hour serum-starved IMIM-PC-2 cells were treated with HGF (20 ng/ml) for different time periods. A: Unpermeabilized, 4% paraformaldehyde-fixed cells were incubated with anti-E-cadherin antibody. Rhodamine-coupled secondary antibody was used to reveal immunoreactivity, and photographs were taken by confocal microscopy. CTL, control cells; HGF, cells treated with HGF for 24 hours. B: Equal amounts of 1% Triton X-100-soluble (s) (50 μg) proteins and equivalent amounts of the insoluble (i) fractions from untreated or HGF-treated cells for the indicated time points were analyzed by Western blotting using anti-E-cadherin antibody.