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. 1998 Jul;153(1):319–329. doi: 10.1016/S0002-9440(10)65574-5

Figure 4.

Figure 4.

Proliferation of DPPIV+ hepatocytes in the liver of retrorsine-treated male DPPIV− mutant rats after partial hepatectomy. A: One day after transplantation of 2 × 10 6 hepatocytes. Several DPPIV+ cells are located near a portal triad, and one DPPIV+ cell is identified more deeply within the hepatic parenchyma. Extensive vacuolization is observed in hepatocytes, which occurs to a varying degree during the first 24 hours after hepatectomy. B: Two weeks posttransplantation. Transplanted hepatocytes show expansion into clusters containing up to 100 or more cells in a cross-sectional area. These clusters, which are more prominent in the periportal spaces, are also present in the midzone but are much smaller in size. Hepatocyte replacement ranged from 15 to 25% in different regions of the liver. C: One month after cell transplantation. Transplanted hepatocytes now form large confluent cell masses representing 50% or more of total hepatocytes. By computer analysis of multiple fields, total hepatocyte replacement was 49% in this animal. D: Two months posttransplantation. Extensive (94%) hepatocyte replacement was observed. Small groups of residual DPPIV− hepatocytes are identified. E: Four months posttransplantation. Near-total (98%) hepatocyte replacement is observed, with only a few remaining endogenous (DPPIV−) hepatocytes at the edges of the lobules and in the central vein region (arrows). F: Nine months posttransplantation. The liver parenchyma is essentially totally replaced by transplanted hepatocytes, which now represent 99% of the hepatocyte mass. Transplanted hepatocytes reform the parenchymal plates with a normal cord structure. G: Nine months posttransplantation in an animal receiving 10,000 cells. In this case, 30 to 50% hepatocyte replacement is observed. H: Six months posttransplantation of 100 DPPIV+ hepatocytes. Clusters containing 200 to 300 DPPIV+ hepatocytes are present in cross sections of the liver. These clusters appear totally integrated into the parenchymal structure. Original magnification: A through H, ×100.

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