Table 3.

Immunohistochemical Identification of sst2A Receptors in Paraffin Sections and Cryostat Sections of Selected Tumors: Comparison with Receptor Autoradiography and in Situ Hybridization

Tumor number	Tumor type	Immunohistochemistry (R2-88)			Receptor autoradiography		In situ hybridization, sst mRNA
		Paraffin*		Cryostat sections
		Cooker	Microwave		OCT-R	SS-28-R
	SS-R positive (sst2)
1	Gastrinoma	+	+	+	+	+	sst2+
2	Bronchial carcinoid	+	+	+	+	+	NT
3	Nonsecreting neuroendocrine carcinoma, duodenum	+	+	+	+	+	NT
4	Intestinal carcinoid	+	+	+	+	+	NT
5	Glucagonoma	+	+	+	+	+	sst2+
6	Insulinoma	+	+	+	+	+	NT
7	Meningioma, fibroblastic and meningothelial	+	+	+	+	+	NT
8	Meningioma, meningothelial	+	+	+	+	+	NT
9	Meningioma, meningothelial	+	+	+	+	+	NT
10	Meningioma, meningothelial	+	+	+	+	+	sst2+
11	Meningioma, fibroblastic and meningothelial	+	+	+	+	+	NT
12	Meningioma, fibroblastic and meningothelial	+	+	+	+	+	NT
13	Pheochromocytoma, adrenal gland	+	+	+	+	+	NT
	SS-R positive (sst2 negative)
14	Insulinoma	−	−	−	−	+	sst3+
15	Prostatic adenocarcinoma, moderately differentiated	−	−	−	−	+	sst1+
16	Prostatic adenocarcinoma, moderately differentiated	−	−	−	−	+	sst1+
17	Prostatic adenocarcinoma, well differentiated	−	−	−	−	+	sst1+
	SS-R negative
18	Pancreatic carcinoma, poorly differentiated	−	−	−	−	−	NT†
19	Pancreatic ductal adenocarcinoma, well differentiated	−	−	−	−	−	NT†
20	Pancreatic ductal adenocarcinoma, moderately differentiated	−	−	−	−	−	NT†
21	Pancreatic adenocarcinoma, poorly differentiated	−	−	−	−	−	NT†
22	Pancreatic ductal adenocarcinoma, poorly differentiated	−	−	−	−	−	NT†
23	Pancreatic adenocarcinoma, moderately differentiated	−	−	−	−	−	NT

*Formalin-fixed, paraffin-embedded section pretreated by boiling in pressure cooker or microwave oven. OCT-R, receptor autoradiography using ¹²⁵I-labeled Tyr³-octreotide; SS-28-R, receptor autoradiography using ¹²⁵I-labeled LTT-SS-28; NT, not tested; SS-R, somatostatin receptors.

^†Shown by Buscail et al¹⁷ to lack sst2 mRNA by RT-PCR.