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. 1998 Jul;153(1):131–139. doi: 10.1016/S0002-9440(10)65553-8

Figure 1.

Figure 1.

Effects of castration on TRPM-2/clusterin mRNA expression in the DLP and VP of NBL rats. Mature male rats were orchiectomized and sacrificed 3 and 7 days after the procedure. Total RNA was isolated from DLPs and VPs of individual animals (n = 3 animals per group). Aliquots (10 μg) of RNA were analyzed by Northern blot analyses using a 32P-labeled TRPM/clusterin probe (pG21–04). 1,12 Blots were washed and rehybridized with a 30-mer oligonucleotide complementary to part of the 18 S rRNA. After each hybridization, x-ray film was exposed by the washed blots at −70°C with intensifying screens for autoradiography. Hybridization signals of the TRPM-2/clusterin mRNA (size, 2.4 kb) and the 18 S rRNA were quantified by densitometric scanning. TRPM-2/clusterin hybridization signals were first normalized with respect to the corresponding 18 S rRNA signal to correct for loading variations. The normalized signal intensities of samples were compared with the contiguous DLP sample prepared from intact rats to arrive at a relative message level. The relative TRPM-2/clusterin mRNA levels in DLPs of intact rats were arbitrarily assigned a value of 1. A: A representative autoradiograph. B: Histograms are relative message levels expressed as group means ± SE derived from three separate experiments.