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. 1998 Aug;153(2):373–379. doi: 10.1016/S0002-9440(10)65581-2

Figure 2.

Figure 2.

REMS-PCR detection of mutant K-ras in DNA from fresh colorectal tumor tissue. Results from six representative colorectal cancers are shown (C1 to C6). DNA from the wild-type cell line K562 analyzed with (+) and without (−) BstN1 in the reaction, and DNA from the heterozygous mutant cell line Calu 1 (Calu) are also shown. Mutant K-ras (81-bp amplicon) is present in three tumors (C1 to C3). All samples contain the template control band (214 bp). Only the K562 DNA analyzed without BstN1 contains the enzyme control band. Absence of this band in the other lanes indicates that the restriction enzyme was active in all other reactions. Products are shown on a 5% NuSieve gel stained with ethidium bromide. MW, phiX174/Hinf.