Table 1.

Oligonucleotides Used in the REMS-PCR Reaction

Primers	Sequence	Significance of amplicon
Diagnostic	5BKIT 5′-TAT AAA CTT GTG GTA GTT GGA CCT	Presence of this amplicon indicates that the template contains mutant K-ras
	3K2 5′-CGT CCA CAA AAT GAT TCT GA
PCR control	5BK38 5′-GTA CAC ATG AAG CCA TCG TAT A	Absence of this amplicon indicates PCR failure
	3K39 5′-CCA CTT GTA CTA GTA TGC CTT AAG
Restriction enzyme control	5BK36 5′-CTA GAA CAG TAG ACA CAA ACC A	Presence of this amplicon indicates BstN1 failure
	3K37 5′-GAT TTT GCA GAA AAC AGA TC

The underscored C in 5BKIT and 5BK36 indicates a mismatch with the K-ras gene.