Agarose gel electrophoresis of RT-PCR-amplified MRP-1/CD9 KAI1/CD82 and ME491/CD63 cDNA. A: MRP-1/CD9 cDNA. B: KAI1/CD82 cDNA. C: ME491/CD63 cDNA. D: β-actin cDNA (internal PCR control). Lane 1, size marker; lane 2, human endothelial cell line ECV304 (positive control); lanes 3 and 4, breast cancers with both MRP-1/CD9- and KAI1/CD82-positive expression; lanes 5 and 6, breast cancers with MRP-1/CD9-positive but KAI1/CD82-reduced expression; lanes 7 and 8, breast cancers with MRP-1/CD9-reduced but KAI1/CD82-positive expression; lanes 9 and 10, breast cancers with both MRP-1/CD9- and KAI1/CD82-reduced expression.