Table 2.
IL-10 and EBER mRNA Expression in Anaplastic T-Cell Lymphomas: Correlation with Cytotoxic Protein Expression by Immunohistochemistry
| Cases | Age/Sex | Histological variant (Kiel/REAL) | Site of biopsy | Other sites of involvement (clinical stage) | IL-10 mRNA expression (positive cells/mm2) | TIA-1 | Granzyme | EBER | EMA | ALK1 | Outcome |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 29 | 66 /M | ALCL T | Node | NA | 37.5 | − | − | − | − | − | NA |
| 30 | 20 /F | ALCL T | Node | IV | 1.4* | ++ | + | − | + | + | CR, AWD (24 months) |
| 31 | 40 /M | ALCL T | Node | III | 98.8 | ++ | ++ | − | − | − | DOD |
| 32 | 40 /M | ALCL T | Node | IV | 105/8.3† | +++ | +++ | − | + | + | CR, AWD (36 months) |
| 33 | 20 /F | ALCL T | Node | II | 80.9 | +++ | +++ | − | + | + | NA |
| 34 | 37 /M | ALCL T | Node | I | 9 | ++ | + | − | + | + | CR |
| 35 | 82 /F | ALCL null | Node | IV | 13.3 | +++ | + | − | − | − | DOD |
| 36 | 38 /M | ALCL null | Node | IV | 87.5/5.5† | + | − | − | ND | − | DOD, 2 weeks |
| 37 | 16 /F | ALCL null | Node | NA | 133.3 | + | +++ | − | ND | + | DOD |
| 38 | 21 /M | ALCL null | Node | II | 46.1 | ++ | +++ | − | ND | − | CR, AWD (16 months) |
| 39 | 11 /M | ALCL null | Node | I | 119.4 | ND | ND | − | + | + | Relapse, 2nd CR Alive (48 months) |
For IL-10, the number of positive cells was estimated per mm 2 of tissue sections. For TIA-1, granzyme B, and EBER, the results were expressed as in Table 1 ▶ .
Abbreviations: REAL, Revised European-American Lymphoma classification; NA, not available; ND, not done; M, male; F, female; AWD, alive without disease; DOD, dead of disease; and CR, complete remission.
*This lymphohistiocytic variant showed only scattered positive cells that could correspond to scattered neoplastic cells.
†Two patterns of expression were observed in the same tissue section; counts were done in two distinct areas of the tissue.