Figure 1.

Examples of multiparameter FCM of DAPI-stained nuclear suspensions obtained from dysplastic areas of human sporadic colorectal adenomas. Top: Forward and side scatter parameters were used to gate out inflammatory cells (corresponding to A2 and B2 subregions) and correct S-phase fraction values among DNA diploid cases. Bottom: Detection of a DNA aneuploid near-diploid peak overlapping with the DNA peak of diploid inflammatory cells. The FCM sorting of an enriched epithelial cell component was done to improve the sensitivity of PCR and sequence-specific oligonucleotide analysis of the K-ras2 spectrum.