Table 2.
DNA Ploidy, Proliferation, and K-ras2
| K-ras2 classes | DI = 1 | DI = 1* | P | Low-S | High-S† | P |
|---|---|---|---|---|---|---|
| 1. Wild type | 70 (80%) | 17 (20%) | 36 (44%) | 45 (56%) | ||
| 2. Mutated | 17 (59%) | 12 (41%) | P12 = 0.02‡ | 18 (64%) | 10 (36%) | P12 = 0.07 |
| 3. G→A transitions | 12 (70%) | 5 (30%) | 12 (75%) | 4 (25%) | P13 = 0.02 | |
| 4. G→C/T tranversions | 5 (42%) | 7 (58%) | P134 = 0.01 | 6 (50%) | 6 (50%) | P134 = 0.08 |
| P14 = 0.003 |
*DI indicates the degree of DNA aneuploidy (DI = 1 for DNA diploidy) evaluated according to multiparametric flow cytometry. Flow cytometry sorting was used to separate epithelial nuclei to be submitted to K-ras2 analysis (see Materials and Methods).
†High-S indicates the values of the S-phase fraction above the median value of 7.3% evaluated among 109 adenomas. S-phase values were corrected by the subtraction of the tissue-infiltrating inflammatory cells (see Materials and Methods). For DNA aneuploid cases, we have evaluated the S-phase fraction of the aneuploid component that mainly corresponds to the epithelial cell compartment.
‡χ2 test-associated probability.