Figure 1.

SDS-PAGE of peritoneal fluid PGs. Peritoneal fluid was passed over an EconoPac Q column, and the nonbound material and GAGs were eluted with increasing concentrations of NaCl (see Materials and Methods). The materials that eluted with 0.4 mol/L NaCl (F1) and 0.6 mol/L NaCl (F2) were separately pooled. Aliquots of F1 (lanes 1 and 2) and F2 (lanes 3 and 4) were incubated with buffer alone (lanes 1 and 3) or chondroitin ABC lyase (lanes 2 and 4) and subjected to SDS-PAGE under reducing conditions on a 3 to 12% gradient gel and stained for GAG with Alcian blue. The prestained molecular mass markers are indicated with arrowheads, and the resolved PGs are indicated with arrows.