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Identification of the 50-kd CSPG as bikunin. Aliquots of the 50-kd CSPG were incubated with buffer alone (lane 1) or chondroitin ABC lyase (lane 2), electrophoresed on 3 to 12% gels, blotted onto nitrocellulose, and probed with an antiserum raised to human serum IαI. The prestained molecular mass markers are indicated with arrowheads and the resolved PGs with arrows. The open arrows indicate the core protein released after incubation with chondroitin ABC lyase.
