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IαI of normal and uremic serum. Aliquots of normal serum (0.5 μl) (lane 1), serum from a patient receiving peritoneal dialysis (0.5 μl) (lane 2), peritoneal fluid from the same patient (5 μl) (lane 3), and serum from a patient with advanced renal failure not receiving CAPD (0.5 μl) (lane 4) were run on 3 to 12% gels, and a Western blot was generated with anti-human IαI antibody. Arrow: Free bikunin; arrowheads: prestained molecular mass markers.
