Table 1.
Inventory of Peritoneal Fluid PGs
| Fraction from ion exchange chromatography | ||
|---|---|---|
| F1 | F2 | |
| PG size | 50 kd | 100 to 200 kd |
| Total GAG | 240 mg | 180 mg |
| CS GAG | 100% | 81% |
| Decorin | ND | +++ |
| Biglycan | ND | + |
| Versican | ND | + |
GAGs were assayed before and after incubation with chondroitin ABC lyase using the Farndale assay 24 with shark CS GAGs as standards. The PG size was determined by SDS-PAGE (see Figure 1 ▶ ). The presence of decorin, biglycan, and versican was determined using Western blot analysis. ND, not detected.