Table 2.

Amino Acid Sequence of the 50-kd proteoglycan

	Amino acid sequence
50 kd PG	AVLPQEEEG[G]GGG
Bikunin*	AVLPQEEEG[ ]GGG
Bikunin†	AVLPQEEEG[S]GGG

The 50-kd CSPG isolated as described in Figure 2 ▶ was incubated with chondroitin ABC lyase and the protein core electrophoresed on a 3 to 12% gradient gel and electroblotted onto a polyvinylidene difluoride membrane for amino acid sequencing using the Edmund N-terminal procedure. Amino acid NH₂-terminal sequences are consistent with the cDNA analysis of bikunin. 57 The failure to recognize serine 10 is due to its glycosylation as the GAG attachment site for this proteoglycan.

*Amino acid NH₂-terminal sequence determined by Enghild et al. 23

^†Amino acid NH₂-terminal sequence determined by a combination of NH₂-terminal sequencing and amino acid analysis. 27