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GST-ACTR (amino acids 621-821) bound to glutathione agarose was incubated with in vitro translated 35S-methionine radiolabeled TRα1 (A) or RARα (B) protein in presence of increasing amounts of cognate agonist. Samples were analyzed using the filter plate method as in Figure 1. Data was fit to a sigmoidal dose response curve with variable slope using GraphPad Prism v. 4.0.
