Table 1. Regression analysis of plate assay.

Best fit values and standard errors from the non-linear regression analysis of the quantification of the interaction of GST-ACTR with varying amount of in vitro translated TRα1 and RARα protein (Figure 2). The data were fit to the equation Y=Bmax^*X/(Kd+X) with the constraints of Bmax<100 and Kd>0. The analysis was done using GraphPad Prism v. 4.0.